Endometriosis Knowledgebase


A repository for genes associated with endometriosis

Results


PMID 23269356
Gene Name DEFB4A
Condition Endometriosis
Association Associated
Population size 50
Population details 50 women including EMS patients undergoing laparoscopic ovarian cystectomy, non-EMS patients undergoing hysterectomy for uterine fibroids
Sex Female
Associated genes hBD-2, TNFA, IL-1b
Expression of human beta-defensin-2 in the eutopic and ectopic endometrial tissues in patients with endometriosis.

Arch Gynecol Obstet. 2013 Jun;287(6):1151-7. doi: 10.1007/s00404-012-2686-7. Epub

Chen, Shu-Qin| Li, Jin-Bo| Jiang, Hong-Ye| Yuan, Li| Niu, Gang| Yao, Shu-Zhong

Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2th Road, 510080 Guangzhou, China. chenshuqin1021@yahoo.com.cn

OBJECTIVE: To investigate the expression of human beta-defensin-2 (hBD-2) in the endometrium of patients with endometriosis (EMS) and explore the potential role of hBD-2 in the pathogenesis of EMS. DESIGN: Prospective controlled study. SAMPLE: 50 women including EMS patients undergoing laparoscopic ovarian cystectomy and non-EMS patients undergoing hysterectomy for uterine fibroids. SETTING: Large university teaching hospital. METHODS: Patients were divided into EMS and non-EMS groups. The gene expressions of hBD-2, interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha in the endometrial tissues of each group were detected with real-time quantitative polymerase chain reaction (PCR), and hBD-2 protein expression with immunohistochemical method. RESULTS: The gene expression levels of hBD-2, TNF-alpha, and IL-1beta as well as the positive expression rate of hBD-2 protein in the ectopic endometrium of EMS patients were significantly higher than those in the eutopic endometrium of EMS and non-EMS patients (all P < 0.05). Correlation analysis showed that the gene expression levels of hBD-2 in the ectopic and eutopic endometrium of EMS patients were positively correlated with the gene expression levels of IL-1beta and TNF-alpha (P < 0.01). CONCLUSION: High levels of hBD-2 gene and protein expressions in the ectopic endometrium of EMS patients may be an important contributor in the pathogenesis of EMS. TNF-alpha and IL-1beta may promote the upregulation of hBD-2 expression.

Mesh Terms: Adult| Endometriosis/*metabolism| Endometrium/*chemistry| Female| Gene Expression| Humans| Immunohistochemistry| Interleukin-1beta/genetics| Prospective Studies| RNA, Messenger/analysis| Real-Time Polymerase Chain Reaction| Tumor Necrosis Facto